For High School Teachers
 
 
  From DNA to Organism: A Study in DNA Function for the High School Biology Classroom
 
 

Materials and vendors

       There are many scientific supply companies that deal with high schools. These are by no means the only places that you can get the needed materials from. I spent significant time on the phone speaking with customer service representatives trying to find the best deal. I realize that the average high school works under a tight budget and some of the equipment can be quite pricey. The good news is that the majority of the items are not consumable and can be bought just once. This is the list of companies that I had luck getting good pricing on quality equipment. The prices are not the exact prices I paid (prices will change) but rather rounded to the nearest dollar just to give an idea of approximate cost.       

      Premodule:

Item Vendor Catalog# Approx Cost
Plant seeds www.tair.org Seed line CS60000 Free to high schools
Pots local garden    
Soil local garden    

      Module 1:

Item Vendor Catalog# Approx Cost
Plant seeds www.tair.org Seed line listed in module Free to high schools
Pots local garden    
Soil local garden    

      Module 2:

DNA extraction

Item Vendor Catalog# Approx Cost
DNA extraction kit www.cartagen.com    
DNA extraction kit www.qaigen.com    
mortar/pestle www.fishersci.com

S337641CR
S337641CRM

10.00
 9.00

1.5ml tubes/500pk asst. colors www.fishersci.com 05 408 137 10.00

2.0ml tubes/500pk asst. colors

www.fishersci.com 05 408 146 11.00
Microtube racks 5pk www.fishersci.com S66127 5.00
2L shallow water bath www.fishersci.com S63075 250.00
Mini-centrifuge      
Micropipette .5-10il www.fishersci.com S3465503 150.00
Tips for .5-10ul in racks www.fishersci.com S66804 20.00
Micropipette www.fishersci.com S346553 150.00
Tips for 10-100ul in racks www.fishersci.com S66806 13.00
Micropipette 100-1000ul www.fishersci.com S346703 150.00
Tips for 100-1000ul in racks www.fishersci.com S66808 18.00

Optional:
Additional pipettes

www.fishersci.com see above  
Vortex mixer

www.fishersci.com

12 813 52 150.00

PCR

Item Vendor Catalog# Approx Cost
Thermalcycler www.sciencekit.com WW7037304 1700.00
.2ul PCR tubes 1000pk www.fishersci.com S65957 12.000

Electrophoresis + Visualization

Item Vendor Catalog# Approx Cost
Micropipettes 3 gel
programmable power supply
www.fishersci.com FB300 300.00

Turn and cast 7X8
electrophoresis rig

www.fishersci.com S64833 200.00
Agarose 100g www.invitrogen 15510019 30.00
Blue juice loading dye www.invitrogen    
TAE buffer 50x www.invitrogen 24710030 18.00

100k DNA standard Syber safe
DNA stain

www.invitrogen    
Portable UV light www.bio-rad.com 166-0500EDU 25.00

Optional

UV light table  
Methylene blue stain (alternative stain) www.wards.com
DNA staining tray  

      Module 3

      Module 4:

Qualitative

Item Vendor Catalob# Approx Cost
1.5ml tubes see DNA extraction above    

Micropipettes
1-10,100-1000

see DNA extraction above    
Petri dishes      
Agar growth media      
K+ additive      

Yeast cultures
(AKT1 +/-)

Uconn biology labs n/a Free to high school

Quantitative

Item Vendor Catalob# Approx Cost
1.5ml tubes see DNA extraction above    

Micropipettes
1-10,100-1000

see DNA extraction above    
Spec 20D+ Spectrophotometer www.fishersci.com 14-385-130 1300.00
Round test tubes for Spec 20D+ www.fishersci.com 07-144-15 50.00

      Module5

      Module6